課程資訊
課程名稱
植物分子生物學研究方法
METHODS IN PLANT MOLECULAR BIOLOGY RESEARCH 
開課學期
97-1 
授課對象
生命科學院  植物科學研究所  
授課教師
吳克強 
課號
PlBio5025 
課程識別碼
B42EU1310 
班次
 
學分
全/半年
半年 
必/選修
選修 
上課時間
星期二2,3,4(9:10~12:10) 
上課地點
生科3C 
備註
本課程以英語授課。與金洛仁、張英峰合開
限學士班四年級以上
總人數上限:30人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/971_method 
課程簡介影片
 
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課程概述

This course covers the topics on the methods and approaches used in plant molecular biology. Emphasis is given on how modern molecular biology tools have been applied to study various plant biology topics. Lectures are combined with literature reading, students’ presentation and discussion. Towards the end of the semester, each student is also required to write a research proposal. 

課程目標
This course covers the topics on the methods and approaches used in plant molecular biology. Emphasis is given on how modern molecular biology tools have been applied to study various plant biology topics. Lectures are combined with literature reading, students’ presentation and discussion. Towards the end of the semester, each student is also required to write a research proposal.  
課程要求
Grades for this course are determined using four criteria: 1) Exams, 2) Paper Presentations, 3) Research Proposal, and 4) Involvement of Discussion. The contribution of each area towards the final grade is as follows: 
預期每週課後學習時數
 
Office Hours
 
指定閱讀
 
參考書目
 
評量方式
(僅供參考)
 
No.
項目
百分比
說明
1. 
Exams  
30% 
 
2. 
Paper Presentations  
30% 
 
3. 
Research Proposal  
30% 
 
4. 
Involvement of Discussion  
10% 
 
 
課程進度
週次
日期
單元主題
第1週
9/16  Molecular Genetic Techniques and Genomics 
第2週
  Molecular Genetic Techniques and Genomics 
第3週
9/30  Regulation of Gene Expression 
第4週
10/07  Regulation of Gene Expression 
第6週
10/21  Introduction, Protein isolation and purification (Sample preparation) 
第7週
10/28  Protein separation and fractionation 
第8週
11/04  Mass spectrometry analysis and protein identification  
第9週
11/18  Post-translational modification 
第10週
11/25  Quantative proteomics 
第11週
2008/12/02  Practical Biological Science Fundamentals 
第12週
2008/12/09  Plant Pathogen Interaction, a Practical Approach 
第13週
2008/12/16  Plant Pathogen Interaction, a Practical Approach 
第14週
2008/12/23  Genomic and Post-Genomic Tools to Advance Plant Molecular Biology 
第15週
2009/01/06  Genomic and Post-Genomic Tools to Advance Plant Molecular Biology